对称二甲基精氨酸(SDMA)是一种甲基化的精氨酸。SDMA来自于L -精氨酸残基的核内甲基化，在蛋白水解后释放到细胞质中。SDMA由肾脏排出。几项研究发现，每3只猫和每10只狗中就有1只可能在它们的一生中患上肾病。SDMA是肾脏功能的早期生物标志物。它与肾小球滤过率(GFR)有很好的相关性。平均而言，肾功能丧失30 - 40%的慢性肾病(CKD)患者中，SDMA增加。然而，直到75%的肾功能丧失，肌酐才会增加。兽医检测SDMA将比肌酐或胱抑素C测试更早地诊断慢性肾脏疾病(CKD)。SDMA对肾功能有特异性。不受肝病、心血管疾病、炎症、内分泌疾病等疾病的影响。SDMA另一个令人兴奋的特点是它也不受肌肉质量的影响，这简化了对瘦的老年动物，特别是猫和患有其他导致肌肉萎缩疾病的动物CKD的诊断和监测。酶免疫测定，用于快速定量测定猫、狗的血清或血浆中的内源性对称二甲基精氨酸（SDMA）Enzyme Immuno Assay for the Fast Quantitative Determination of Endogenous Symmetric Dimethylarginine (SDMA) in Serum or Plasma of Dogs and Cats规格：微孔板（12 x 8测定，单裂孔），预包被SDMAFormat：Microtiter plate (12 x8 determinations, single break apart wells), coated with SDMA疾病：猫、狗慢性肾脏疾病Diseases：Chronic Kidney Disease in cats and dogs临床领域：兽医Clinical Fields：Veterinary Medicine检测原理：竞争性的SDMA-ELISA试剂盒采用微孔板检测。SDMA与微孔板的固相结合。样品中的SDMA被酰基化，并与固相结合的SDMA竞争固定数量的兔抗SDMA抗血清结合位点。当系统处于平衡状态时，通过洗涤去除游离抗原和游离抗原-抗血清复合物。通过抗兔/过氧化物酶检测与固相SDMA结合的抗体。在450 nm处检测底物TMB /过氧化物酶反应。与固相SDMA结合的抗体量与样品中SDMA浓度成反比。The competitive SDMA-ELISA uses the microtiter plate format. SDMA is bound to the solid phase of the microtiter plate. SDMA in the samples is acylated and competes with solid phase bound SDMA for a fixed number of rabbit anti-SDMA antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase SDMA is detected by anti-rabbit / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase SDMA is inversely proportional to the SDMA concentration of the sample. 特异性交叉反应：SDMA 100